SRA

We use the a porcine genome-scale CRISPR/Cas9 knockout (PigGeCKO) library (Zhao et al., 2020) to identify key host factors facilitating TGEV infection in porcine cells. Overall design: We generated a PigGeCKO cell collection (in the PK-15 background) as previously described and performed a genome-wide loss-of-function genetic screen.Briefly, we conducted three rounds of TGEV challenge, employing untreated PK-15-Cas9 cells as a negative control.